Fluxor herg assay h9c2

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WebFluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium … WebThis also means that the assay is carried out in a physiological, normal-chloride saline. in this article, the authors describe how they used Bacmam gene delivery to express Kv7.2 and 7.3 (KcnQ), Kir2.1, or Kv11.1 (herg) potassium ion channels in u2-os cells. using these cells, they ran the Fluxor™ assay to identify and

Assessing the translatability of In vivo cardiotoxicity mechanisms …

WebThe FluxOR™ Potassium Ion Channel Assay provides a concentrated thallium solution and all necessary buffers, allowing maximum target flexibility and ease of operation in a homogenous format that has been demonstrated for use with cells stably expressing hERG, as well as our BacMam-hERG delivery and expression reagent (Catalog number B10019 ... Webthe FluxOR™ II Green Potassium Ion Channel Assay (ThermoFisher Cat# F20016) and HEK293-hERG cells (human embryonic kidney cell line stably expressing hERG). … trusted traders welwyn garden city

Thallium Flux Assay Protocol Download Table - ResearchGate

WebAfter the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by a thallium stimulus buffer addition (10 … WebThree K+ Channels, hERG, Kir2.1, and Kv7.2, were differentially expressed in U-2 OS cells using BacMam technology. Following an overnight incubation cells were dye loaded using FluxOR then incubated with compounds or controls. Each of the four compound plates (Tocris) was tested against the three cell lines hERG, Kir2.1, and Kv7.2. WebThe FluxOR assay was performed using the washing method recommended by the supplier, and bottom fluorescence intensity was assessed by end-point readout in the SynergyH1 microplate reader … trusted trader south ayrshire

Frontiers Early identification of hERG liability in drug …

Cell-Based Potassium Ion Channel Screening Using the FluxOR™ Assay …

WebThe FluxOR™ II Green Potassium Ion Channel Assay is the next-generation solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR II Green assay takes … philip ross hondaWebDec 15, 2010 · In the present study, we describe a high-capacity, 384- and 1,536-well plate, functional thallium flux assay for the hERG channel that fulfills these criteria. This assay was optimized and validated using different structural classes of hERG inhibitors. philip ross construction

"WebThe hERG assay, an in vitro electrophysiologic assay, has become routine since its development in the 1990s. It is one of the recommended studies in the ICH S7B regulatory guideline. This is a voltage clamp assay where a cultured mammalian cell (usually human embryonic kidney, or HEK cell) is transfected with hERG DNA. " - Fluxor herg assay h9c2

Fluxor herg assay h9c2

Frontiers Development of a High-Throughput Flow Cytometry …

WebMar 17, 2024 · The FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit hERG channel activity. This chapter describes a cell-based hERG channel inhibition assay that has been optimized and performed in a 1536-well plate format. WebMar 1, 2010 · The FluxOR assay was able to identify several known specific inhibitors of Kv7.2/7.3 or hERG, highlighting its potential to identify novel and more efficacious small-molecule modulators.

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WebFluxOR™ Differentially Identifies K+ Channel Antagonists Using FDSS6000 Reaction Time (sec) Z' Score FDSS7000EX Effect of reaction time on z' scores using hERG cells and … WebAfter the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by a thallium stimulus buffer addition (10 μL/well) and ...

WebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye … WebJul 16, 2024 · H9C2 cells were subcultured under anoxic conditions to mimic myocardial hypoxia and subsequently treated with salidroside. Whole cell patch clamp was …

WebThe FluxOR™ Potassium Ion Channel Assay is a solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR™ assay takes … WebSep 6, 2013 · For ATP depletion assays, H9C2 and RCM’s cells were plated in 96 well plates per the manufacturer’s protocol for 24 hr prior to treatments. For gene expression …

Webs of hERG inhibitors determined in separate tests. N=4. FLIPR Potassium Kit-4 -2 0 2 0 1 3 Dofetilide Terfenadine Pimozide Log[inhibitor] mM) FluxOr Kit-4 -2 0 2 0.0 0.2 0.4 0.6 0.8 1.0 Dofetilide Terfenadine Pimozide Log[inhibitor] mM Compound) IC 50 (nM) FLIPR Potassium Assay 50) FluxOR Assay Dofetalide 39 53 Pimozide 135 195 Terfenadine …

WebApr 8, 2024 · ATP produced by H9c2 cells was assessed by the bioluminescence assay kit (Beyotime Biotechnology, Shanghai, China). In brief, the concentration of ATP was assessed by adding 20 μL of the supernatant into 100 μL of luciferase reagent; the luciferase present in the reagent catalyzes the production of luminescence from ATP and … trusted trader window companyWebThe Predictor™ hERG kit from Invitrogen is a homogeneous fluorescent assay that uses a simple add-and-read format. The assay is based on the principle of fluorescence … trusted traders south ayrshire councilWebHere, we sought to develop an optical high-throughput assay to monitor surface and total hERG protein expression that would be useful to discern mechanisms underlying LQT2 … philip ross facebookWebFor high-throughput screening of potassium ion channel and transporter activities we use the FluxOR™ II Green Potassium Ion Channel Assay (Thermo Fisher Cat# F20016) and … trusted traders suffolk county councilWebThe FluxOR™ assay has been proven identified several known specific inhibitors of Kv7.2/7.3 or hERG, highlighting its potential to identify novel and more efficacious small-molecule modulators. This assay is based on the permeability of potassium channels to thallium. To run the assay, cells are loaded with non-fluorescent, thallium-specific ... philip rossignol re think bio cleanWebFluxOR Assay Kit (Life Technologies, Carlsbad, CA) following the manufacturers protocol. • Fluorescence signals were elicited by 1mM Tl + and 10 mM K + and detected on FLIPR … philip rossiWebApr 1, 2010 · To validate the feasibility of the FluxOR™ assay as a screening tool for potassium channels, we went on to test the pharmacology of known potassium channel modulators identified as hits in Figure 2.Based on their well-characterized inhibition of hERG or Kv7.2/7/3, haloperidol, linopiridine, XE991, and cisapride were chosen for further … trusted traders which review